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Radiation biophysics

The group consists of Marie Davidkova, Vaclav Stepan, Katerina Pachnerova Brabcova, Anna Michaelidesova, Jana Vachelova and Jana Konirova. We deal with biological effects of ionizing radiation and radiotherapy in following topics:
  • damage to DNA, proteins and DNA-protein complexes
  • cellular radiobiology
  • biological effects of charged particles, XUV and UV
  • development of theoretical model RADAMOL (physical and chemical stage of radiation interaction with biological targets in water)
  • characterization of radiotherapeutic sources
  • fragmentation of primary ions

Publications


Relative biological effectiveness in a proton spread-out Bragg peak formed by pencil beam scanning mode,
A. Michaelidesová, J. Vachelová, M. Puchalska, K. Pachnerová Brabcová, V. Vondráček, L. Sihver, M. Davídková, Australasian Physical and Engineering Science in Medicine (2017) doi: 10.1007/s13246-017-0540-8

Breaking DNA strands by extreme-ultraviolet laser pulses in vacuum,
E. Nováková, L. Vyšín, T. Burian, L. Juha, M. Davídková, V. Múčka, V. Čuba, M.E. Grisham, S. Heinbuch, J.L. Rocca, Physical Review W 91 (2015) 042718.

Development of a compact laser-produced plasma soft X-ray source for radiobiology experiments,
D. Adjei, M.G. Avele, P. Wachulak, A. Bartnik, L. Wegrzynski, H. Fiedorowicz, L. Vyšín, A. Wiechec, J. Lekki, W.M. Kwiatek, L. Pina, M. Davídková, L. Juha, Nuclear Instruments and Methods in Physics Research Section B 364 (2015) 27-32.

Track structure modeling in liquid water: A review of the Geant4-DNA very low energy extension of the Geant4 Monte Carlo simlation toolkit,
M.A. Bernal, M.C. Bordage, J.M.C. Brown, M. Davídková, E. Delage, Z. El Bitar, S.A. Enger, Z. Francis, S. Guatelli, V.N. Ivanchenko, M. Karamitros, I. Kyriakou, L. Maigne, S. Meylan, K. Murakami, S. Okada, H. Payno, Y. Perrot, I. Petrovic, Q.T. Pham, A. Ristic-Fira, T. Sasaki, V. Štěpán, H.N. Tran, C. Villagrasa, S. Incerti, Physica Medica 31 (2015) 861-874.

Mass production of fluorescent nanodiamonds with a narrow emission intensity distribution,
J. Stursa, J. Havlik, V. Petrakova, M. Gulka, J. Ralis, V. Zah, Z. Pulec, V. Stepan, S. A. Zargaleh, M. Ledvina, M. Nesladek, F. Treussart, P. Cigler, Carbon 96 (2016) 812-818.

Dose-dependent micronuclei formation in normal human fibroblasts exposed to proton radiation,
A.V. Litvinchuk, J. Vachelová, A. Michaelidesová, R. Wagner, M. Davídková, Radiation and Environmental Biophysics 54 (2015) 327-334.

Proton-induced direct and indirect damage of plasmid DNA,
L. Vyšín, K. Pachnerová Brabcová, V. Štěpán, P. Moretto-Capelle, B. Bugler, G. Legube, P. Cafarelli, R. Casta, J.P. Champeaux, M. Sence, M. Vlk, R. Wagner, J. Štursa, V. Zach, S. Incerti, L. Juha, M. Davídková, Radiation and Environmental Biophysics 54 (2015) 343-352.

Contribution of indirect effects to clustered damage in DNA irradiated with protons,
K. Pachnerová Brabcová, V. Štěpán, M. Karamitros, M. Karabín, P. Dostálek, S. Incerti, M. Davídková, L. Sihver, Radiation Protection Dosimetry 166 (2015) 44-48.

RADAMOL tool: Role of radiation quality and charge transfer in damage distribution along DNA oligomer,
V. Štěpán, M. Davídková, The European Physical Journal D 68 (2014) 240.

Clustered DNA damage on sub-cellular level: effect of scavengers,
K. Pachnerová Brabcová, L. Sihver, N. Yasuda, V. Štěpán, M. Davídková, Radiation and Environmental Biophysics 53/4 (2014) 705-712.

Chromatin differentiation of white blood cells decreases DSB damage induction, prevents functional assembly of repair foci, bbut has no influence on protrusion of heterochromatic DSBs into the low-dense chromatin,
M. Falk, E. Lukasova, I. Falkova, L. Stefancikova, L. Jezkova, A. Bacikova, M. Davidkova, A. Boreyko, E.A. Krasavin, S. Kozubek, Journal of Radiation Research 55/1 (2014) 81-82.

Heterochromatinization associated with cell differentiation as a model to study DNA double strand break induction and repair in the context of higher-order chromatin structure,
M. Falk, E. Lukášová, L. Štefančíková, E. Baranová, I. Falková, L. Ježková, M. Davídková, A. Bačíková, J. Vachelová, A. Michaelidesová, S. Kozubek, Applied Radiation and Isotopes 83 (2014) 177-185.

Simulating radial dose of ion tracks in liquid water simulated with Geant4-DNA: A comparative study,
S. Incerti et al., Nuclear Instruments and Methods in Physics Research B 333 (2014) 92-98.

Function of chromatin structure and dynamic in DNA damage, repair and misrepair: gamma rays and protons in action,
L. Ježková, M. Falk, I. Falková, M. Davídková, A. Bačíková, L. Štefančíková, J. Vachelová, A. Michaelidesová, E. Lukášová, A. Boreyko, E. Krasavin, S. Kozubek, Applied Radiation and Isotopes 83 (2014) 128-136.

Micronuclei in human peripheral blood lymphocytes exposed to mixed beams of X-rays and alpha particles,
E. Staaf, K. Brehwens, S. Haghdoost, S. Nievaart, K. Pachnerová Brabcová, J. Czub, J. Braziewicz, A. Wojcik, Radiat. Environ. Bioph. 51(3) (2012) 283-293.

Characterization of a setup for mixed beams exposure of cells to 241Am alpha particles and X-rays,
E. Staaf, K. Brehwens, S. Haghdoost, K. Pachnerová Brabcová, J. Czub, J. Braziewicz, S. Nievaart, A. Wojcik, Rad. Prot. Dos. (2012).

Molecular scale track structure simulations in liquid water using the Geant4-DNA Monte-Carlo processes,
Z. Francis, S. Incerti, R. Capra, B. Mascialino, G. Montarou, V. Štěpán, C. Villagrasa, Applied Radiation and Isotopes 69(1) (2011) 220-226.

Radiation damage to DNA in DNA–protein complexes,
M. Spotheim-Maurizot, M. Davídková, Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 711 (2011) 41-48.

Radiation damage to DNA-protein complexes,
M. Spotheim-Maurizot, M. Davídková, COST Chemistry CM0603–MELUSYN Joint Meeting IOP Publishing, Journal of Physics: Conference Series 261 (2011) 012010.

Effects of gamma irradiation on the DNA-protein complex between the estrogen response element and the estrogen receptor,
V. Štísová, S. Goffinont, M. Spotheim-Maurizot, M. Davídková, Radiat. Phys. Chem. 79 (2010) 880-889.

Lethal events in V79 cells irradiated by low-energy protons and correlations with distribution patterns of energy deposition, radical concentration and DNA damage,
M. Davídková, P. Kundrát, V. Štěpán, Z. Palajová, L. Judas, Applied Radiation and Isotopes 67 (2009) 454-459.

Impact of oxygen concentration on yields of DNA damages caused by ionizing radiation,
V. Štěpán, M. Davídková, Radiation Damage in Biomolecular Systems, Journal of Physics: Conference Series 101 (2008) 012015.

RADACK, a tool for studying the radiolysis of DNA-protein complexes,
M. Davídková, M. Spotheim-Maurizot, COST P9 monograph, Canopus/Springer Verlag 2007.

Significance of 8-oxoG in the spectrum of DNA damages caused by ionizing radiation of different quality,
V. Štěpán, M. Davídková, Radiat. Prot. Dosim. 121(4) (2006) 113-115.

Theoretical modeling of radiolytic damage of free DNA bases and within DNA macromolecule,
V. Štěpán, M. Davídková, Radiat. Prot. Dosim. 121(4) (2006) 110-112.

Radiation damage to DNA-protein specific complexes: Estrogen response element – estrogen receptor complex,
V. Štísová, S. Goffinont, M. Spotheim-Maurizot, M. Davídková, Radiat. Prot.
Dosim. 121(4) (2006) 106-109.

Modification of DNA radiolysis by DNA-binding proteins. Structural aspects,
M. Davídková, V. Štísová, S. Goffinont, N. Gillard, B. Castaing, M. Spotheim-Maurizot, Radiat. Prot. Dosim. 121(4) (2006) 100-105.

The comparison of calculated and experimental microdosimetric distributions for carbon ions,
Z. Palajová, F. Spurný, M. Davídková, Radiat. Prot. Dosim. 121(4) (2006) 491-493.

Microdosimetry distributions for 40-200 MeV protons,
Z. Palajová, F. Spurný, M. Davídková, Radiat. Prot. Dosim. 121(4) (2006) 376-381.

 
 
Jana working in laminar flow box
 Jana working in laminar flow box.
 
Anna looking through microscope
  Anna is looking through the microscope onto irradiated fibroblasts.
 
Richard with ionization chamber
 Richard checks ionization chamber before irradiation of biological samples.
 
The work space in laboratory
 The working area in the laboratory.
 
Agarose gels
 DNA samples after electrophoresis.

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